![]() For aggregated protein samples, DLS results can show that the aggregate distribution in the sample is polydisperse, but it is not feasible to obtain detailed quantitative information on monomer and aggregate content. Particle concentrations cannot be obtained by DLS. For protein monomers and aggregates, a size range from about 1 nm to several µm can be covered. The particle size range of DLS depends on the properties of the analyzed species, such as refractive index or density, as well as the surrounding formulation, mainly the viscosity. Dynamic light scattering is a technique in physics that can be used to determine the size distribution profile of small particles in suspension or polymers. The technique can also be employed for the analysis of colloidal systems, such as liposomes, nanoparticles, polymers and virus-like particles. The Dynamic Light Scattering Technique was used to determine the size, shape and diffusion coefficient of nanoparticle. Dynamic light scattering (DLS), which is also known as photon correlation spectroscopy (PCS) or quasi-elastic light scattering (QLS), is a spectroscopy method used in the fields of chemistry, biochemistry, and physics to determine the size distribution of particles (polymers, proteins, colloids, etc.) in solution or suspension. DLS can determine the hydrodynamic size of protein monomers, small aggregates in the nanometer range and partially also particles in the high nanometer/low micrometer range. ![]() Based on intensity fluctuations of laser light scattered by the molecules/particles, moving in Brownian motion, the diffusion coefficient is determined and converted to particle size via the Stokes-Einstein equation. ![]()
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